Recombinant protein production processes are actually a key focus of the bio-industry. Despite this, the purification of these proteins is still awaiting improvements because this step represents a very expensive cost of production for a low specificity.
This invention concerns a new protein tag to be linked to a recombinant protein of interest in order to purify it by affinity chromatography. This system allows a one-step column purification with a much higher purity rate and a similar yield to conventional methods. In addition, elution is carried out with inexpensive and non-toxic lactose.
The effectiveness of the new tag has been validated in different prokaryotic-type protein production systems (E. coli) and is currently being studied in eukaryotic systems (HEK-type mammalian cells particularly).
Examples of proteins produced by this method (Kriznik et al. Biotechnology Journal 2018): an enzyme (thioredoxin Trx1), a transcription factor (ESRα), a receptor (TREM1) usually produced in an insoluble manner (solubility obtainment: Carasco et al. Cellular and Molecular Immunology 2018)...
MARKETS & APPLICATIONS
Biotechnology: purification / detection of recombinant proteins
Technology validated in prokaryotic system and under development in eukaryotic system
Laboratory "Ingénierie Moléculaire & Physiopathologie Articulaire" (IMoPA) University of Lorraine - CNRS
Patent registered on May 13th, 2016 (PCT/FR2017/051140)
Business Development Manager
+33 (0)7 76 16 66 90