Visualisation of collagen matrix by non-destructive macroscopy

Presentation

The imaging platform PTIBC IBISA has developed a multimodal macroscope, capable of performing wide-field imaging and reconstituting the 3D network of the collagen matrix, without sample contact. It becomes possible to highlight the effect of collagen remodeling, without using exogenous contrast agents. This new, non-invasive, and non-intrusive imaging tool brings us closer to physiological reality. Sterility is guaranteed for in-vitro samples. It is also possible to observe ex-vivo samples during several days, while maintaining them alive under perfusion. Biases related to observation of thin sections from different samples are therefore avoided.

Competitive advantages

• Rapid observation of sample surfaces of several cm2 in one pass using classical macroscopy (~ 40 min for 2 cm2);
• Observation of interest zones with classical microscopy;
• High resolution observation of precise points with confocal macroscopy;
• Analysis of sample structure in depth (up to 500-600 μm) by multiphoton macroscopy, while preserving life.
  

Applications/Markets

• Control of active substance action on collagen matrix;
• Control of collagen matrix lesions.
  

Intellectual Property 

• Patent FR1154545;
• Readiness level: functional prototype;
• Search for license for macroscope commercialization or service for industrial groups or public laboratories.

Laboratory

• Plateforme d’Imagerie et de Biophysique Cellulaire (PTIBC)