Genetically engineered PRORP protein for the development of RNA-Virus resistant plants

CONECTUS ALSACE



15 Juillet 2015

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Fields

Biology / Medical

Sectors

Health
  • Novel genetical approach offering plants a universal resistance against TLS-RNA-viruses (27 viruses/ 8 families)
  • Vast possibilities of [Virus/plants] couples & Market opportunities

TECHNOLOGY

  • PRORP proteins family are newly discovered RNAse P activity enzymes, located in organels, able to cleave tRNA & tRNA-like-sequences (TLS).
  • TLS are constituents of 3’-extremity of some virus RNA, Necessary to use the cellular machinery and proliferate.
  • Idea : Cytosolic PRORP mutant (CytoRP) can be obtained by deletion/mutation of the localization sequence. Consequently, CytoRP can exhibit a RNAse-P activity in the cytoplasm, avoiding virus RNA proliferation.

 

APPLICATION

  • TLS RNA-viruses resistant plants generation (8 families, 27 virus, including TYMV, TMV, CMV …)
  • Applicable to any vegetable crops.
  • GMO & Non-GMO transformations possible

 

INNOVATION ADVANTAGES

  • Not transgenic : CYTORP DNA results from a DNA sequence sequence deletion/mutation of PRORP
  • Multiple resistances in 1 transformation
  • Low risk of resistance development thanks to the new resistance Mode of action
  • CRISPR-Cas9 method possible and currently investigated

 

DEVELOPMENT STATUS

  • Cucumber CYTORP ensymetically active on virus cleavage
  • In vivo POC in Arabidopsis = established virus resistant plants by CYTORP transgenic expression
  • Gene edited plants ongoing (Arabidopsis)

 

Partnership : out-licensing

 

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