2C-DLS - Detection of analytes in solution by using nanoparticles as light scattering enhancers

BACKGROUND

Analytes detection (e.g. proteins, nucleic acids, DNA, bacterial fragments…) is important for both molecular biology research and medical applications.

However, the existing detection methods show a low signal to noise ratio, that results in a limited sensitivity and a low specificity.

2C-DLS is an original method and system for detection the presence of analytes in solution, with an excellent signal to noise ration and a very good specificity.

HOW IT WORKS

The method takes advantage of using two different nano-probes, used to bound to one targeted analyte.

As each probe has a specific optical response, the method enables to specifically detect the aggregate formed by the analyte combined with, at least, first and second probes.

When light scattered by the probes are detected simultaneously over time, it confirms the presence of the analyte in the solution.

KEY BENEFITS vs. STATE OF THE ART

• Obtained a unique signature for one or more specifics analytes at same times.
• Excellent signal to noise ratio, with exclusion of signals from isolated probes.
• The signal is a thousand times more intense than fluorescence.
• Permits a compact and low cost device by avoiding sensitive detectors or high aperture microscope objectives.
• Needs only a small observation volume, typically between 1 to 10 μm3, without purification steps.
• Allows to detect small concentration, up to pico or femtomolar.

DEVELOPMENT STATUS

Pre-industrial prototype

APPLICATIONS

Detection and analysis of biomolecules: DNA, proteins, bacterial fragments…