Bioprinting is a growing technology with various applications in making functional tissue constructs in order to replace injured or diseased tissues. Bioinks display a crucial role in this area. Indeed, its properties such as mechanical, rheological, and biological properties are essential to ensure the functionality of the bio printed tissues and organs.
Fibrinogen and fibrin bioinks are currently used to fabricate functional tissue constructs for the replacement of damaged tissues due to their beneficial roles in wound healing. They are biocompatible, biodegradable, and non-immunogenic, and induce cell attachment, proliferation, and ECM formation.
However, fibrin shaping has so far faced major issues related to the required addition of thrombin to fibrinogen to trigger fibrillogenesis. Indeed, its enzyme-induced self-assembly mechanism makes it difficult to shape, which limits the possibility to recreate 3D structures mimicking targeted tissues and organs.
On the other hand, fibrinogen production requires many steps to lead to a 3D structure and limit the production of fiber-based scaffolds. In addition, the mechanical properties of such scaffolds are not necessarily optimized to be used in various clinical applications such as tissue reconstruction
The present technology is about two-steps methods for producing fibrin and fibrinogen gel.
Both methods are based on incubation or sterilization process and lead to fibrinogen or Fibrin gel will improved properties in terms of mechanical and rheological properties.
Wound Healing Skin tissue engineering Vascular tissue engineering