BACKGROUND

Nosocomial infection is a major public health stake

• 6% of medical devices responsible for nosocomial infections

High needs of innovation because of:

• Difficulty to formulation wide spectrum action products

• Complexity of use (several products and frequent use) for hospital staff

• Current use of enzymes as detergents but not as anti-microbials

TECHNOLOGY

Mechanism of action: Lactonase able to hydrolyze lactones molecules involved in the inter-bacteria communication and virulence factors (quorum sensing inhibition)

 Activity: Ability to inhibit biofilm formation and virulence factor production by P. aeruginosa

 Activity spectrum on Gram(-), P. Aeruginosa, Acinetobacter

 No activity loss after coating on biomaterials

 Toxicology/safety data: No toxicity observed up to 10mg/ml (rat model)

KEY BENEFITS

• Highly thermostable(Tm = 106 °C)

• Stable more than 1 year in liquid buffer at room temperature and more than 5 years after freeze-drying

• Not denatured when coupled to detergent

• Potential synergy with detergents: “two-in-one” product (washing+disinfection) for medical device

• Eco-friendly

APPLICATIONS

Hospital disinfection:

• Medical devices detergents

• Surface and floor disinfection

• Air disinfection - Airway decontamination