New non-retractable biomaterial for cell culture

The biomaterial is based on a synthetic interpenetrating polymer network (IPN) of a UV controlled co-reticulated proteic polymer (fibrin based) and synthetic polymer (based on polyethylen glycol) with a fibrin gel obtained in the same medium through an enzymatic reaction. This biomaterial does not shrink over time and allows 2D or 3D cellular colonization making it a perfect in vitro extra-cellular matrix model for cell based assays. This biomaterial can take any wanted shape and size, especially it can be poured at the bottom of culture plate wells without retraction of the gel matrix including High Throughput Screening (HTS) plates. This biomaterial can be easily produced following a “one pot-one shot” process enabling a constant batch to batch quality. In addition it can also be easily handled and stored as it can be lyophilisated and sterilized. This biomaterial is an ideal matrix for cellular growth using different gel thickness on all formats of cell culture plates (even HTS) allowing the development of customized in vitro cell based assays.

Competitive advantages : 

• Non retractable matrix
• 2D and 3D matrix for cell culture
• Easy to produce, handle and store (sterilization and lyophilisation)
• Compatible with high throughput screening tests

Applications :

• 2D or 3D matrix for in vitro cell based assays
• Research or preclinical customized in vitro screening tool

Keywords : Synthetic biomaterial, Copolymer, Fibrin gel, Non-retractable matrix, 2D and 3D cell culture, In vitro cell based assay