New self-inducible heterologous protein expression systems

SAYENS



26 Juillet 2018

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Fields

Biology / Medical

Sectors

Health

CONTEXT 

The limitations of current recombinant protein production systems include: the use of a transcription inducer and specific medium, low yield, high costs and complex industrial scaling.

DESCRIPTION

To overcome these difficulties, we have developed a new self-inducible recombinant protein production system. Our technology is based on a SILEX (Self-InducibLe EXpression) plasmid able to transfer to a bacteria the ability to induce autonomously heterologous protein expression without the use of a chemical inducer and a specific culture medium. SILEX can be used from microliter to large production scales and is compatible with a wide range of existing plasmids.

COMPETITIVE ADVANTAGES

  • Protein production without cell monitoring and without the use of chemical inducer and specific culture medium
  • Easy and fast screening of protein production conditions (medium, temperature, plasmid) in order to optimize its yield
  • Application from microliter to large production scales with time and cost savings
  • It allows the production of complex proteins (poorly soluble), which is currently difficult with existing systems

 

MARKETS & APPLICATIONS

Biotechnology : production of recombinant proteins at laboratory (research community and commercial suppliers) or industrial scale (pharmaceutical, agri-food industry...)

 

DEVELOPMENT STAGE

Technology tested i) on over 40 heterologous proteins, ii) with over 10 media and iii) on 96-well plates with volumes as low as 25μl

 

RESEARCH TEAM

"Centre des Sciences du Goût et de l'Alimentation" (Dijon, France)

 

INTELLECTUAL PROPERTY

Patent WO2016046137 (09/2014): granted in the USA, European grant in progress in France and Belgium

 

TARGET PARTNERSHIP

Patent licensing or services in protein production

CONTACT

Ludmila MONTEIRO

Business Development Manager

+33 (0)6 31 10 21 21

ludmila.monteiro@sayens.fr

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